Species Database

Trypanosoma cruzi Pangenome

Complete genome assemblies across multiple clades and hybrid lineages, with telomere-to-telomere resolution and comprehensive annotation.

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Genome Strains

The T. cruzi pangenome comprises strains organized into four discrete clades (A–D) and hybrid lineages (BC). Each strain page provides genome browsers, dotplots, Circos visualizations, and gene annotations. Strain Dm28c was sequenced T2T in our lab (Greif et al., 2026) and two haplotypes were available.

Navigate to each clade via the Pangenome Figure above or through the dropdown menu in the navigation bar.

Trypanosoma cruzi pangenome figure Clade A Clade B Clade D Clade C Hybrids Clade A Clade B Clade C Clade D Hybrids
Figure 1. Pangenome overview of Trypanosoma cruzi. The figure illustrates the pan-genomic structure organized by clade and hybrid lineage. Adapted from Berná et al. (2021 ).
Clade A 6 strains
Clade B 2 strains
Clade C 3 strains
Clade D 3 strains
Hybrids 5 strains

Publications

1
Greif, G., Chiribao, M., Diaz-Viraque, F. et al.
The complete genome of Trypanosoma cruzi reveals 32 chromosomes and three genomic compartments.
BMC Genomics (2026). https://doi.org/10.1186/s12864-025-12482-0
2
Díaz-Viraqué F, Greif G, Berná L, Robello C.
Nanopore Long Read DNA Sequencing of Protozoan Parasites: Hybrid Genome Assembly of Trypanosoma cruzi.
Methods Mol. Biol. (2021). https://doi.org/10.1007/978-1-0716-1681-9_1
3
Díaz-Viraqué F, Chiribao ML, Libisch MG, Robello C.
Genome-wide chromatin interaction map for Trypanosoma cruzi
Nat. Microbiol. (2023). https://doi.org/10.1038/s41564-023-01483-y
4
Díaz-Viraqué F, Pita S, Greif G, de Souza RCM, Iraola G, Robello C.
Nanopore Sequencing Significantly Improves Genome Assembly of the Protozoan Parasite Trypanosoma cruzi.
Genome Biol. Evol. (2019). https://doi.org/10.1093/gbe/evz129
5
Díaz-Viraqué F, Pita S, Greif G, de Souza RCM, Iraola G, Robello C.
Transcriptome Studies in Trypanosoma cruzi Using RNA-seq.
Methods Mol. Biol. (2019). https://doi.org/10.1007/978-1-4939-9148-8_3
6
Berná L, Rodriguez M, Chiribao ML, Parodi-Talice A, Pita S, Rijo G, Alvarez-Valin F, Robello C.
Expanding an expanded genome: long-read sequencing of Trypanosoma cruzi.
Microb. Genom. (2018). https://doi.org/10.1099/mgen.0.000177
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